CRL-7601 Hs 865.Sk 人皮膚成纖維細胞，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|。細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和最優(yōu)培養(yǎng)條件!


CRL-7601 Hs 865.Sk 人皮膚成纖維細胞 的詳細介紹

CRL-7601 Hs 865.Sk 人皮膚成纖維細胞



ATCC® Number:

CRL-7601™

Price:








Designations:

Hs 865.Sk



Biosafety Level:

1



Shipped:

frozen



Medium & Serum:

See Propagation



Growth Properties:

adherent



Organism:

Homo sapiens (human)



Morphology:

fibroblast





Source:

Organ: skin
Disease: normal



Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.







Age:

59 years



Gender:

female



Ethnicity:

Caucasian



Comments:

The line was established from apparently normal tissue from a patient who had adenocarcinoma of the stomach.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.



Propagation:

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C



Subculturing:

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.



Preservation:

culture medium 95%; DMSO, 5%



Related Products:

Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020